Histology and Histomorphometry Core
Core Mission
The Goals of the P30 Histology Core are to:
- Provide guidance and mentorship to all investigators interested in the design and execution of studies required to analyze skeletal phenotypes
- Provide consultation, mentorship, and routine services at highly discounted prices to junior investigators
- Provide initial consultation and subsidized services to established investigators
- Establish new protocols for phenotyping, including immunohistochemistry (IHC), lineage tracing, and non-traditional histomorphometry
- Provide on-going formal education and training on all services offered by the Core
- Allow for close interactions with the imaging, bone cells, and RNA Seq cores to assure coordinated studies permitting imaging, histology, cell isolation, and RNA analyses on skeletal elements from a singles subject. To coordinate these services please contact the relevant core directors.
Services
- Consultation with Director or technicians available by appointment
- Initial consultation for assistance in experimental design or protocol trouble shooting (IHC) provided at no charge
- Paraffin: processing, embedding, sectioning
- Decalcified Frozen Sections: embedding and sectioning
- Non-decalcified Frozen Sections: embedding and sectioning
- Resin/MMA: processing, embedding, and sectioning
- Staining: H&E, Trichrome, Safranin O
- Histomorphometry: Traditional and non-traditional parameters, OARSI scoring for articular cartilage
Equipment and Resources
- Tissue processor for paraffin blocks
- Paraffin embedding station
- Microtomes
- Cryostats
- Fluorescent and brightfield microscopy
- Osteomeasure for histomorphometry
- Low speed saw and grinder/polisher for resin/MMA sections
*P30 Discounts available for Full Members, Associate Members, and Members in Training