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Protocols

Bone Cells

  • Alizarin Red Staining Protocol
  • Bone marrow cells
  • Bone Marrow Macrophages
  • CRISPR Cas9 PX458
  • Ear MSC Isolation and Culture
  • OCY454 Osteocytic Cell Line
  • Osteoblasts differentiations
  • Primary Chondrocyte Culture
  • Primary Osteoblasts from Calvaria

Imaging and Biomechanics

Please complete the attached form for sample processing and upload to core’s online requisition form: Spreadsheet file

  • Description of services, fees, and policies
  • Harvesting mouse long bones and vertebrae

    Contact MICROCTCORE@PARTNERS.ORG for protocol

  • JBMR guidelines for the assessment of bone microstructure using microCT
  • Preparation for MicroCT scanning

Histology & Histomorphometry

Tissue Preparation

  • 4% PFA and 10% formalin fixative protocol
  • Calcein injection protocol
  • Consultation Service
  • Decalcification of bone
  • Embedding and sectioning of decalcified frozen sections Protocol
  • Embedding and sectioning of fresh, non-decalcified frozen sections of mineralized tissue
  • Paraffin processing, embedding, sectioning
  • Resin processing, embedding and sectioning of non-decalcified mineralized tissue
  • Staining: H&E, Trichrome, Safranin O, Von Kossa

Immunohistochemistry

  • Active beta-catenin
  • CD31
  • Cleaved Caspase 9
  • Endomucin
  • IHC phospho SMAD3
  • IHC SOX9 milipore
  • Klotho
  • MMP13
  • NOGGIN (R&D AF719)
  • Osteocalcin
  • Perilipin
  • pERK12IHC
  • pS6
  • pSMAD159
  • Runx2 (Cell Signaling)
  • Sclerostin IHC
  • vegf IHC protocol

In Situ hybridization probes

  • ISH plasmids

Questions? Please contact csrmail@partners.org

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Become a Member

The benefits of membership in P30 include subsidized rates in the CSR cores, experienced faculty to assist with projects, networking with other bone scientists, and much more.

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News

  • SEPTEMBER CSR NewsletterSeptember 2023 - 11:25 am

    Click here to view September CSR Newsletter.

  • CORT Pilot & Feasibility Award – LOI due 9/15August 2023 - 1:02 pm

    CORT Pilot & Feasibility Award The Center of Research Translation on Osteoporosis Bone Anabolic Therapies (CORT), based in the Endocrine Unit at MGH and funded by the National Institute of […]

  • AUGUST CSR NewsletterAugust 2023 - 12:59 pm

    Click Here to view the August CSR Newsletter

  • JULY CSR NewsletterJuly 2023 - 4:51 pm

    Click here to access the July CSR Newsletter

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Contact us

Center for Musculoskeletal Research
Endocrine Unit
Massachusetts General Hospital
50 Blossom Street, Thier 1101
Boston, MA 02114-2696

Telephone: 617-726-3967
Fax: 617-726-7543
Email: csrmail@partners.org

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