Bone Cells
Cell Signaling Assays
- cAMP (direct RIA method)
- cAMP (real-time glosensor reporter assay)
- Fura-2 iCa++
- IP1 (Cis-Bio HTRF assay)
- IP3 (3H-myoinositol labeling method)
- Mixed Array (SABio Cignal 45 pathway array)
- p-ERK-1/2
- Wnt/β-catenin (SABio- TCF/LEF-Luc reporter)
- β-arrestin recruitment (DiscoverX, PathHunter recruitment assay)
Imaging and Biomechanics
Please complete the attached form for sample processing and upload to core’s online requisition form: Spreadsheet file
Histology & Histomorphometry
Tissue Preparation
- 4% PFA and 10% formalin fixative protocol
- Consultation Service
- Decalcification of bone
- Embedding and sectioning of decalcified frozen sections
- Embedding and sectioning of fresh, non-decalcified frozen sections of mineralized tissue
- Frozen decalcified and undecalcified tissue submission instructions
- MMA plastics tissue submission instructions
- Paraffin processing, embedding, sectioning
- Resin processing, embedding and sectioning of non-decalcified mineralized tissue
- Staining: H&E, Trichrome, Saffranin O, Von Kossa
Immunohistochemistry
In Situ hybridization probes
MGH CSR Internal Protocol
Internal Resource for CSR core technicians:
https://partnershealthcare.sharepoint.com/sites/mghcsradmin
Questions? Please contact csrmail@partners.org