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Protocols

Bone Cells

  • Alizarin Red Staining Protocol
  • Bone marrow cells
  • Bone Marrow Macrophages
  • CRISPR Cas9 PX458
  • Ear MSC Isolation and Culture
  • OCY454 Osteocytic Cell Line
  • Osteoblasts differentiations
  • Primary Chondrocyte Culture
  • Primary Osteoblasts from Calvaria

Cell Signaling Assays

  • cAMP (direct RIA method)
  • cAMP (real-time glosensor reporter assay)
  • Fura-2 iCa++
  • IP1 (Cis-Bio HTRF assay)
  • IP3 (3H-myoinositol labeling method)
  • Mixed Array (SABio Cignal 45 pathway array)
  • p-ERK-1/2
  • Wnt/β-catenin (SABio- TCF/LEF-Luc reporter)
  • β-arrestin recruitment (DiscoverX, PathHunter recruitment assay)

Imaging and Biomechanics

Please complete the attached form for sample processing and upload to core’s online requisition form: Spreadsheet file

  • Description of services, fees, and policies
  • Harvesting mouse long bones and vertebrae

    Contact MICROCTCORE@PARTNERS.ORG for protocol

  • JBMR guidelines for the assessment of bone microstructure using microCT
  • Preparation for MicroCT scanning

Histology & Histomorphometry

Tissue Preparation

  • 4% PFA and 10% formalin fixative protocol
  • Calcein injection protocol
  • Consultation Service
  • Decalcification of bone
  • Embedding and sectioning of decalcified frozen sections Protocol
  • Embedding and sectioning of fresh, non-decalcified frozen sections of mineralized tissue
  • MMA plastics tissue submission instructions
  • Paraffin processing, embedding, sectioning
  • Resin processing, embedding and sectioning of non-decalcified mineralized tissue
  • Staining: H&E, Trichrome, Saffranin O, Von Kossa

Immunohistochemistry

  • Active beta-catenin
  • CD31
  • Cleaved Caspase 9
  • Endomucin
  • IHC phospho SMAD3
  • IHC SOX9 milipore
  • Klotho
  • MMP13
  • NOGGIN (R&D AF719)
  • Osteocalcin
  • Perilipin
  • pERK12IHC
  • pS6
  • pSMAD159
  • Runx2 (Cell Signaling)
  • Sclerostin IHC
  • vegf IHC protocol

In Situ hybridization probes

  • ISH plasmids

MGH CSR Internal Protocol

Internal Resource for CSR core technicians:
https://partnershealthcare.sharepoint.com/sites/mghcsradmin

Questions? Please contact csrmail@partners.org

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The benefits of membership in P30 include subsidized rates in the CSR cores, experienced faculty to assist with projects, networking with other bone scientists, and much more.

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News

  • Journal Club ArticlesApril 2021 - 3:34 pm

    Click the title to go to PDF Association of Potent and Very Potent Topical Corticosteroids and the Risk of Osteoporosis and Major Osteoporotic Fractures. Presented on April 9, 1:00pm by Sherri-Anne Burnett-Bowie. Association of Osteonecrosis of the Jaw With Zoledronic Acid Treatment for Bone Metastases in Patients With Cancer. Presented on April 9, 1:00pm by […]

  • Now Accepting Applications for Pilot & Feasibility Award 2021March 2021 - 1:50 pm

    The Center for Skeletal Research (CSR), based in the Endocrine Unit at MGH, and funded by the National Institute of Arthritis and Musculoskeletal Diseases (NIAMS) is announcing a request for Pilot and Feasibility Research proposals LETTER OF INTENT DUE MONDAY APRIL 5, 2021 5PM EST Click Here for more information

  • Summer Lecture Series RecordingsDecember 2020 - 2:43 pm

    Please follow these links to listen to the recordings of the CSR 2020 Summer Lecture Series “Bone cells and their functions” by Paola Divieti Pajevic, MD, PhD “Gene editing and cell signaling” by Marc Wein, M.D., Ph.D. and Kathy Tang “Osteoporosis and Space Flight Induced Bone Loss” by Mary Bouxsein, PhD “Vitamin D” by Marie […]

  • Postdoctoral Fellow Position – Evolutionary and Developmental Genetics Laboratory, Dr. Terence D. CapelliniDecember 2020 - 12:01 pm

    The Department of Human Evolutionary Biology at Harvard University invites applications for a Postdoctoral Fellow in the Evolutionary and Developmental Genetics Laboratory of Dr. Terence D. Capellini.  The focus of the position will be to study the genetic and developmental basis of human-specific adaptations and disease.  While the lab primarily concentrates on skeletal adaptations in […]

Upcoming Events

  1. UCSF Crosstalk in Osteoarthritis: Within and Beyond the Joint

    April 16 @ 11:00 am - 3:30 pm
  2. BUSDM Department of Translational Dental Medicine Seminar Series

    April 17 @ 12:00 pm - 1:00 pm
  3. CSR Inter-Organ Communication Series

    April 23 @ 3:30 pm - 5:00 pm

View All Events

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Contact us

Center for Skeletal Research
Endocrine Unit
Massachusetts General Hospital
50 Blossom Street, Thier 1101
Boston, MA 02114-2696

Telephone: 617-726-3967
Fax: 617-726-7543
Email: csrmail@partners.org

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