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Protocols

Bone Cells

  • Alizarin Red Staining Protocol
  • Bone marrow cells
  • Bone Marrow Macrophages
  • CRISPR Cas9 PX458
  • Ear MSC Isolation and Culture
  • OCY454 Osteocytic Cell Line
  • Osteoblasts differentiations
  • Primary Chondrocyte Culture
  • Primary Osteoblasts from Calvaria

Cell Signaling Assays

  • cAMP (direct RIA method)
  • cAMP (real-time glosensor reporter assay)
  • Fura-2 iCa++
  • IP1 (Cis-Bio HTRF assay)
  • IP3 (3H-myoinositol labeling method)
  • Mixed Array (SABio Cignal 45 pathway array)
  • p-ERK-1/2
  • Wnt/β-catenin (SABio- TCF/LEF-Luc reporter)
  • β-arrestin recruitment (DiscoverX, PathHunter recruitment assay)

Imaging and Biomechanics

Please complete the attached form for sample processing and upload to core’s online requisition form: Spreadsheet file

  • Description of services, fees, and policies
  • Harvesting mouse long bones and vertebrae

    Contact MICROCTCORE@PARTNERS.ORG for protocol

  • JBMR guidelines for the assessment of bone microstructure using microCT
  • Preparation for MicroCT scanning

Histology & Histomorphometry

Tissue Preparation

  • 4% PFA and 10% formalin fixative protocol
  • Calcein injection protocol
  • Consultation Service
  • Decalcification of bone
  • Embedding and sectioning of decalcified frozen sections Protocol
  • Embedding and sectioning of fresh, non-decalcified frozen sections of mineralized tissue
  • MMA plastics tissue submission instructions
  • Paraffin processing, embedding, sectioning
  • Resin processing, embedding and sectioning of non-decalcified mineralized tissue
  • Staining: H&E, Trichrome, Saffranin O, Von Kossa

Immunohistochemistry

  • Active beta-catenin
  • CD31
  • Cleaved Caspase 9
  • Endomucin
  • IHC phospho SMAD3
  • IHC SOX9 milipore
  • Klotho
  • MMP13
  • NOGGIN (R&D AF719)
  • Osteocalcin
  • Perilipin
  • pERK12IHC
  • pS6
  • pSMAD159
  • Runx2 (Cell Signaling)
  • Sclerostin IHC
  • vegf IHC protocol

In Situ hybridization probes

  • ISH plasmids

MGH CSR Internal Protocol

Internal Resource for CSR core technicians:
https://partnershealthcare.sharepoint.com/sites/mghcsradmin

Questions? Please contact csrmail@partners.org

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Become a Member

The benefits of membership in P30 include subsidized rates in the CSR cores, experienced faculty to assist with projects, networking with other bone scientists, and much more.

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News

  • Two post-doctoral associate positions – 1) Cartilage Regeneration & Osteoarthritis and 2) Cancer Basic or Translational Research, Boston University – Dr. Bais LabJuly 2022 - 11:48 am

    Two post-doctoral associate positions available at Bais Lab related to 1) cartilage regeneration and osteoarthritis 2) cancer basic or translational research Location: Boston University, Bais lab.   Excellent opportunities for candidates to learn and apply innovative thinking in target focused research with cutting-edge tools including signaling pathways to translational animal models.  We are interested in […]

  • Research Technician Position, Endocrine Unit – Dr. Marie Demay LabApril 2022 - 2:44 pm

    A Research Laboratory Technician position is available in the laboratory of Dr. Marie Demay in the Endocrine Unit MGH. The Research Laboratory Technician I is a full-time position, and the primary responsibility is to assist in ongoing research studies under the supervision of a Principal Investigator. This includes collection of and accurate recording of procedures […]

  • MGH Endocrine Unit Faculty PositionFebruary 2022 - 4:56 pm

    Faculty Position Instructor/Assistant Professor  Department of Medicine, Massachusetts General Hospital, Harvard Medical School  The Endocrine Unit at Massachusetts General Hospital and Harvard Medical School invites applications for a Junior Faculty position at the Instructor/Assistant Professor level. Outstanding scientists in the field of basic/translational research will be considered. Candidates with a research program focused on hormonal […]

  • MTA Samples for Bone Cells Core Service RequestsJanuary 2022 - 12:34 pm

    Sample MTAs: Sample MTA for Human Cells – Bone Cells Core Sample MTA for OCY454 – Bone Cells Core   Blank MTAs to be filled by requestors: Blank MTA for Human Cells – Bone Cells Core Blank MTA of OCY454 – Bone Cells Core   For service requests, complete these forms and email to:  BoneCell@bu.edu […]

Upcoming Events

  1. BWH Orthopaedic Research Special Seminar @4pm EG

    September 7 @ 4:00 pm - 5:00 pm
  2. CSR Visiting Speaker Series – “Osteocytes, PTH receptor signaling and bone anabolism” Teresita Bellido, PhD

    November 18 @ 4:00 pm - 5:00 pm

View All Events

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Contact us

Center for Skeletal Research
Endocrine Unit
Massachusetts General Hospital
50 Blossom Street, Thier 1101
Boston, MA 02114-2696

Telephone: 617-726-3967
Fax: 617-726-7543
Email: csrmail@partners.org

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