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  • Leptin R. Tomato

    Lineage Tracing of bone marrow adipocytes showing a positive red tomato signal. (White arrows ) in green Lipitox stained adipocytes. Blue represents DAPI stained nuclei.

  • Composite RGB

    Immunostaining for CA3 (green) in osteocyclic cell line Ocy454. Blue represents DAPI stained nuclei.

  • Fura 2-Ca complex

    Fura-2 radiometric fluorescence assay of PTH-induced intracellular calcium signaling in HEK293/THR1 (GP-2.3) cells performed by the Cell Signaling Assay Core.

  • 3D Femur Trabecular Bone Reconstructions

    Representative 3D reconstructions of the trabecular bone in the distal femoral metaphysis of two genotypes of mice at four time points. This type of figure is great for demonstrating trabecular bone phenotypes in presentations and publications.

  • Osmium tetroxide staining for marrow adipose tissue analysis

    3D Reconstruction of the marrow adipose tissue (MAT) in the proximal and distal tibia of a control and treated mouse. The adipose tissue is stained with osmium tetroxide (dark grey in the images), which is radiopaque and can be images with µCT. Osmium tetroxide staining can be quantified with µCT to measure the effect of study parameters on MAT volume.

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Our Mission

The Center for Skeletal Research will further the discovery of the causes and therapies for skeletal diseases through the provision of core facilities and other programs designed to make the work of a broad group of investigators more efficient, interdisciplinary, innovative, and interactive. By making this research more effective, advances in understanding and therapy of osteoporosis and other bone disease will occur more quickly.

Research Cores

  • Arthritis and Joint Structure Core

    Arthritis and Joint Structure Core

    The core aims to address a need to support CSR musculoskeletal researchers to expand their studies from their original research toward the understanding of the mechanism of joint diseases, including osteoarthritis, osteoimmunology and joint structure pathology.

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  • Bone Cells Core

    Bone Cells Core

    We provide high quality skeletally derived osteoblasts, osteoclasts, bone marrow stromal cells and osteocytes, as well as protocols and training for isolating, characterizing and isolating skeletally derived cells. We also develop novel techniques for isolating and studying bone cells.

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  • Bone RNAseq Core

    Bone RNAseq Core

    The RNA seq core provides help and advice with designing of bulk tissue and single cell RNA sequencing experiments, help with tissue and cell preparation, access to space and equipment, expertise for performing bulk RNA extractions from bone and cartilage tissue and computational and statistical interpretation of RNA sequencing data.

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  • Cell Signaling Assay Services

    Cell Signaling Assay Services

    Provides/Enables Assays for Cell Signaling Pathways
    cAMP, IP3, iCa, ERK-1/2, Multi-Array, 1,25 Dihydroxy Vitamin D, 25-Hydroxy Vitamin D, cAMP RIA, CTX-1,FGF-23, Fura-2 iCa++, Glosensor cAMP, IP1, P1NP, p-ERK-1/2, PTH.
    Formats: Luminescence, RIA, ELISA, HTRF
    Kit assays on a per-sample cost-basis.

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  • Directed Differentiation Core Services

    Directed Differentiation Core Services

    NEW CORE!
    The Craft lab develops and uses directed differentiation protocols for various skeletal and joint lineages from pluripotent stem cells (PSCs), including both embryonic stem cells and induced pluripotent stem cells, to study cell and tissue development and disease pathology.

    Learn More
  • Histology & Histomorphometry Services

    Histology & Histomorphometry Services

    Close interactions with the Imaging, Bone Cells and Signaling Cores will assure coordinated studies permitting imaging, histology, cell isolation, RNA and signaling analyses on skeletal elements from a single subject.

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  • Imaging and Biomechanical Testing Services

    Imaging and Biomechanical Testing Services

    High-resolution micro-computed tomography (µCT) provides provides non-destructive, quantitative and qualitative assessment of trabecular and cortical bone microarchitecture.

    Learn More

Remember to acknowledge the Center for Skeletal Research if you received funding or services from our cores, see How to Cite P30.
P30 AR066261 – Core Services 2014 – May 31, 2019
P30 AR075042 – Core Services June 1, 2019 – May 31, 2024


Funded by P30 AR075042.

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The benefits of membership in P30 include subsidized rates in the CSR cores, experienced faculty to assist with projects, networking with other bone scientists, and much more.

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News

  • NEW: Directed Differentiation Core ServicesJanuary 2023 - 10:11 am

    PI: April Craft, PhD Assistant Professor of Orthopedic Surgery, BCH, HMS; Affiliate Faculty, Stem Cells & Regenerative Biology; Principal Faculty, Harvard Stem Cell Institute; Co-Director, Musculoskeletal Disease Program, HSCI Email:  april.craft@childrens.harvard.edu      […]

  • NEW CORE: Arthritis and Joint Structure CoreJanuary 2023 - 3:57 pm

    Innovation Award for use of new Arthritis and Joint Structure Core Click Here to access the application. Email filled out applications to CSRmail@partners.org. This award will provide up to $5000 […]

  • CSR Mentee Survey for CSR TraineesNovember 2022 - 12:12 pm
  • Northeastern University Open Rank: Assistant/Associate/Professor – Healthy Aging, Department of BiologyOctober 2022 - 3:31 pm

    Click Here for the application link and the job description

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Center for Skeletal Research
Endocrine Unit
Massachusetts General Hospital
50 Blossom Street, Thier 1101
Boston, MA 02114-2696

Telephone: 617-726-3967
Fax: 617-726-7543
Email: csrmail@partners.org

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