Bone Cells Core
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GOLDMAN SCHOOL OF DENTAL MEDICINE, BOSTON UNIVERSITY
BONE CELLS CORE
Director: Paola Divieti Pajevic, MD PhD; pdivieti@bu.edu
Associate Director: Louis Gerstenfeld, PhD; lgersten@bu.edu
Core Manager: Chris Constantinou; BoneCell@bu.edu
Mission
- Provide high quality skeletally derived (osteoblasts, osteoclasts, bone marrow stromal cells and osteocytes)
- Provide and process clinical bone samples (whole bone marrow, bone marrow stromal cells, bone chips)
- Provide protocols and training for isolating, characterizing and isolating skeletally derived cells
- Develop novel techniques for isolating and studying bone cells
Click Here to access the Bone Cell Core Database – CSR Member Access Only (please email CSRmail@partners.org to request access)
Services
- Primary osteoblasts derived from wild type (WT) or genetically modified animals
- Calvarial osteoblasts
- Long bone osteoblasts
- Bone marrow derived stromal cells (BMSC) from wild type (WT) or genetically modified animals
- Bone marrow derived macrophages (osteoclast precursors)
- Chondrocytes derived from wild type (WT) or genetically modified animals
- Isolation of Fluorescently tagged cells
- Isolation of cells from human specimen (IRB approval)
- Training/assistance in flow cytometry and sorting of specific bone cell populations
- Loading (fluid flow) and unloading experiments using bone cells (from the Core) or customer derived cells
- CRISPR Cas9 PX458
- Ear MSC Isolation and Culture
- Lentiviral-mediated silencing
- Ocy454 osteocytic cell line (MTA required)
- Click here for MTA instructions & samples