GOLDMAN SCHOOL OF DENTAL MEDICINE, BOSTON UNIVERSITY

BONE CELLS CORE

Director:  Paola Divieti Pajevic, MD PhD; pdivieti@bu.edu 

Associate Director:  Louis Gerstenfeld, PhD; lgersten@bu.edu

Core Manager:  Chris Constantinou; BoneCell@bu.edu

Mission

1. Provide high quality skeletally derived (osteoblasts, osteoclasts, bone marrow stromal cells and osteocytes)
2. Provide and process clinical bone samples (whole bone marrow, bone marrow stromal cells, bone chips)
3. Provide protocols and training for isolating, characterizing and isolating skeletally derived cells
4. Develop novel techniques for isolating and studying bone cells

Click Here to access the Bone Cell Core Database – CSR Member Access Only (please email CSRmail@partners.org to request access)

Services

• Primary osteoblasts derived from wild type (WT) or genetically modified animals
• Calvarial osteoblasts
• Long bone osteoblasts
• Bone marrow derived stromal cells (BMSC) from wild type (WT) or genetically modified animals
• Bone marrow derived macrophages (osteoclast precursors)
• Chondrocytes derived from wild type (WT) or genetically modified animals
• Isolation of Fluorescently tagged cells
• Isolation of cells from human specimen (IRB approval)
• Training/assistance in flow cytometry and sorting of specific bone cell populations
• Loading (fluid flow) and unloading experiments using bone cells (from the Core) or customer derived cells
• CRISPR Cas9 PX458
• Ear MSC Isolation and Culture
• Lentiviral-mediated silencing
• Ocy454 osteocytic cell line (MTA required)
  • Click here for MTA instructions & samples