Core Mission

The Core provides assistance in implementing Light-Seq technology in your hands. Light-Seq is a spatial transcriptomic technique that can be implemented without the need to purchase expensive equipment. For this reason it is widely accessible and potentially applicable to any tissue and or species of interest.

Services

• Light-Seq applicability (with preliminary experiment)
• Consulting on study design and sample preparation
• Guidance on data analysis

Equipment and Resources

• We can provide Light-Seq reagents
• We can mediate access to confocal microscopy facility
• We can facilitate data storage and analysis on the HMS O2 computer cluster

 

Learn more

Original publication – describes the capabilities and limitations of the approach

Original protocol – lists all necessary equipment, reagents, and step-by-step instructions

Original data analysis pipeline – scripts for each step from handling raw reads to visualizing read counts to differential gene expression analyses

Light-Seq explained

 

Contact us for any inquiry

All advices provided here are based upon the original source material linked above. We are purely optimizing this technique on different tissues with a special emphasis on skeletal ones – Giulia.Montagna@childrens.harvard.edu, Katherine.Woronowicz@childrens.harvard.edu, josephine.nwokedi@childrens.harvard.edu, and Matthew.Warman@childrens.harvard.edu

 

*P30 Discounts available for Full Members, Associate Members, and Members in Training