Name* First Last Bone Cell ServicesPlease answer the following questions. This is not binding, it will just give us an idea of potential usage.1. Mouse cells: Osteoblast, BMM, MBSC, MSC, chondrocyte, primary adipocytes Once-twice a month Once-twice a year Once None 2. Mouse cells: Cells lines (Ocy454, MC3T3, ROS, RAW 264.7) Once-twice a month Once-twice a year Once None 3. Human samples: Fresh or frozen bone marrow, bone cells or MSCs Once-twice a month Once-twice a year Once None 4. Human samples: Immortalized human bone cells Once-twice a month Once-twice a year Once None 5. Specialized cultures: 3D cultures, perfusion, fluid flow Once-twice a month Once-twice a year Once None 6. Genetic tools: Validated gRNA and shRNAs. Lenti-Cre (murine) Once-twice a month Once-twice a year Once None Analytical Assay Services7. Consultation for study design Once-twice a month Once-twice a year Once None 8. Cell Signaling: cAMP (RIA or glosensor), intracellular calcium, Ip1, pERK1/2 Once-twice a month Once-twice a year Once None 9. Blood or urine markers: Ca, Pi, Creatinine, BUN, cAMP, CTX-1,CTX-11, PiNp, 25D, 1,25D Bone Alk phos, TRAP5b, osteocalcin, PTH, FGF23 Once-twice a month Once-twice a year Once None RNA Sequencing Services10. Consultation for study design Once-twice a month Once-twice a year Once None 11. RNA sequencing: Library prep, bioinformatics Once-twice a month Once-twice a year Once None 12. RNA sequencing: Single Cell RNA seq Bioinformatic Once-twice a month Once-twice a year Once None Imaging & Biomechanical Testing Services13. MicroCT of murine bone Once-twice a month Once-twice a year Once None 14. Other MicroCT services (Write in below)15. Marrow adiposity by osmium tetroxide Once-twice a month Once-twice a year Once None 16. Mechanical testing of murine bones Once-twice a month Once-twice a year Once None Please provide comments or recommend additional services below:
