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NEW: Directed Differentiation Core Services

NEW: Directed Differentiation Core Services

January 2023/in News /by Melissa Machado
PI: April Craft, PhD

Assistant Professor of Orthopedic Surgery, BCH, HMS; Affiliate Faculty, Stem Cells & Regenerative Biology; Principal Faculty, Harvard Stem Cell Institute; Co-Director, Musculoskeletal Disease Program, HSCI

Email:  april.craft@childrens.harvard.edu              Longwood office: (617) 919-4495

Overview

The Craft lab develops and uses directed differentiation protocols for various skeletal and joint lineages from pluripotent stem cells (PSCs), including both embryonic stem cells and induced pluripotent stem cells, to study cell and tissue development and disease pathology.  Dr. Craft has been trained in the derivation of various mesoderm and endoderm lineages, with a focus on mesoderm-derived chondrocytes, articular and growth plate cartilage tissues, and cardiac lineages, and has new technologies emerging for tendon-ligament fibroblasts.  These existing protocols provide access to primitive streak mesoderm, endoderm, as well as paraxial mesoderm and mesenchymal-like progenitors.

            Dr. Craft and her lab are available to discuss investigator-driven projects, experimental design, and provide hands-on training for generating working stocks of human iPSCs/ESCs and mouse PSCs, directed differentiations, deriving appropriate culture conditions and media compositions to support novel protocols, and troubleshooting existing protocols.  We also have experience with culturing and manipulating primary human skeletal cells (primarily chondrocytes and connective tissue lineages) and mouse embryonic limb bud micromass cultures. Our lab has purchased lot-tested reagents in bulk and we have a supportive infrastructure in place so that variability in PSC differentiations is minimized.

Example Services

  • Consultation with Dr. Craft (required)
    • 1 hour complementary to CSR members and trainees; over 1 hour – $100/hr
  • Training or production in Craft lab
    • IPSC/ESC expansion and creation of master stock and working stocks (~50-100 vials total) ~ $1450
    • Human iPSC/ESC directed differentiation (full-length experiment) ~ $2000-2600
      • Imaging: EVOS fluorescent microscope, Leica bright field microscope, Zeiss confocal
      • Flow cytometry available ($45/hr + antibody) in BCH core via Craft lab
      • Other analyses such as RNA/histo at investigator’s expense
    • Mouse ESC/iPSC expansion and/or differentiation ~ $700-900
    • Mouse limb bud micromass training ~ $550-900 (timed pregnant mice can be purchased if needed)
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News

  • NEW: Directed Differentiation Core ServicesJanuary 2023 - 10:11 am

    PI: April Craft, PhD Assistant Professor of Orthopedic Surgery, BCH, HMS; Affiliate Faculty, Stem Cells & Regenerative Biology; Principal Faculty, Harvard Stem Cell Institute; Co-Director, Musculoskeletal Disease Program, HSCI Email:  april.craft@childrens.harvard.edu      […]

  • NEW CORE: Arthritis and Joint Structure CoreJanuary 2023 - 3:57 pm

    Innovation Award for use of new Arthritis and Joint Structure Core Click Here to access the application. Email filled out applications to CSRmail@partners.org. This award will provide up to $5000 […]

  • CSR Mentee Survey for CSR TraineesNovember 2022 - 12:12 pm
  • Northeastern University Open Rank: Assistant/Associate/Professor – Healthy Aging, Department of BiologyOctober 2022 - 3:31 pm

    Click Here for the application link and the job description

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